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cav3 2  (Alomone Labs)


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    Structured Review

    Alomone Labs cav3 2
    All experiments in ( a – c ) are performed at E11.5 at the ileo-cecal junction. Error bars are ±SD. a CA change relative to control before drug application for EDTA (2 mM, n = 6), GdCl3 (100 µM, n = 6), 2-APB (100 µM, n = 5), ryanodine (10 µM, n = 5). b CA change for nifedipine (Nif, 10 µM, n = 5), ruthenium red (RuR, 100 µM, n = 6), Z944 at 1 µM ( n = 7), 10 µM ( n = 7), 50 µM ( n = 9), ascorbic acid (AA, 1 mM, n = 13) and SAK3 at 1 µM ( n = 6), 10 µM ( n = 6). c CA change for NPPB (100 µM, n = 11), NFA (50 µM, n = 9) and DIDS (500 µM, n = 5). Two-tailed p -values for (a-c) are calculated from the Student t-test. Sox10 and <t>CaV3.1</t> ( d <t>),</t> <t>CaV3.2</t> ( e ) and CaV3.3 ( f ) IHCs of ENCCs migrating from an intestinal explant on a substrate. All three T-type Ca 2+ channels are expressed by ENCCs, but not by the surrounding mesenchymal cells. The small bright green dots are AF647 µ-beads fluorescing in the same range as the T-type Ca 2+ channel secondary antibody. They were added in the overlying collagen gel for another experiment (see Fig. ).
    Cav3 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cav3 2/product/Alomone Labs
    Average 93 stars, based on 89 article reviews
    cav3 2 - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Endothelin-3 and T-type Ca 2+ channels drive enteric neural crest cell calcium activity, contractility and migration"

    Article Title: Endothelin-3 and T-type Ca 2+ channels drive enteric neural crest cell calcium activity, contractility and migration

    Journal: Nature Communications

    doi: 10.1038/s41467-025-68121-5

    All experiments in ( a – c ) are performed at E11.5 at the ileo-cecal junction. Error bars are ±SD. a CA change relative to control before drug application for EDTA (2 mM, n = 6), GdCl3 (100 µM, n = 6), 2-APB (100 µM, n = 5), ryanodine (10 µM, n = 5). b CA change for nifedipine (Nif, 10 µM, n = 5), ruthenium red (RuR, 100 µM, n = 6), Z944 at 1 µM ( n = 7), 10 µM ( n = 7), 50 µM ( n = 9), ascorbic acid (AA, 1 mM, n = 13) and SAK3 at 1 µM ( n = 6), 10 µM ( n = 6). c CA change for NPPB (100 µM, n = 11), NFA (50 µM, n = 9) and DIDS (500 µM, n = 5). Two-tailed p -values for (a-c) are calculated from the Student t-test. Sox10 and CaV3.1 ( d ), CaV3.2 ( e ) and CaV3.3 ( f ) IHCs of ENCCs migrating from an intestinal explant on a substrate. All three T-type Ca 2+ channels are expressed by ENCCs, but not by the surrounding mesenchymal cells. The small bright green dots are AF647 µ-beads fluorescing in the same range as the T-type Ca 2+ channel secondary antibody. They were added in the overlying collagen gel for another experiment (see Fig. ).
    Figure Legend Snippet: All experiments in ( a – c ) are performed at E11.5 at the ileo-cecal junction. Error bars are ±SD. a CA change relative to control before drug application for EDTA (2 mM, n = 6), GdCl3 (100 µM, n = 6), 2-APB (100 µM, n = 5), ryanodine (10 µM, n = 5). b CA change for nifedipine (Nif, 10 µM, n = 5), ruthenium red (RuR, 100 µM, n = 6), Z944 at 1 µM ( n = 7), 10 µM ( n = 7), 50 µM ( n = 9), ascorbic acid (AA, 1 mM, n = 13) and SAK3 at 1 µM ( n = 6), 10 µM ( n = 6). c CA change for NPPB (100 µM, n = 11), NFA (50 µM, n = 9) and DIDS (500 µM, n = 5). Two-tailed p -values for (a-c) are calculated from the Student t-test. Sox10 and CaV3.1 ( d ), CaV3.2 ( e ) and CaV3.3 ( f ) IHCs of ENCCs migrating from an intestinal explant on a substrate. All three T-type Ca 2+ channels are expressed by ENCCs, but not by the surrounding mesenchymal cells. The small bright green dots are AF647 µ-beads fluorescing in the same range as the T-type Ca 2+ channel secondary antibody. They were added in the overlying collagen gel for another experiment (see Fig. ).

    Techniques Used: Control, Two Tailed Test



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    cav3 2  (Alomone Labs)
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    Alomone Labs cav3 2
    All experiments in ( a – c ) are performed at E11.5 at the ileo-cecal junction. Error bars are ±SD. a CA change relative to control before drug application for EDTA (2 mM, n = 6), GdCl3 (100 µM, n = 6), 2-APB (100 µM, n = 5), ryanodine (10 µM, n = 5). b CA change for nifedipine (Nif, 10 µM, n = 5), ruthenium red (RuR, 100 µM, n = 6), Z944 at 1 µM ( n = 7), 10 µM ( n = 7), 50 µM ( n = 9), ascorbic acid (AA, 1 mM, n = 13) and SAK3 at 1 µM ( n = 6), 10 µM ( n = 6). c CA change for NPPB (100 µM, n = 11), NFA (50 µM, n = 9) and DIDS (500 µM, n = 5). Two-tailed p -values for (a-c) are calculated from the Student t-test. Sox10 and <t>CaV3.1</t> ( d <t>),</t> <t>CaV3.2</t> ( e ) and CaV3.3 ( f ) IHCs of ENCCs migrating from an intestinal explant on a substrate. All three T-type Ca 2+ channels are expressed by ENCCs, but not by the surrounding mesenchymal cells. The small bright green dots are AF647 µ-beads fluorescing in the same range as the T-type Ca 2+ channel secondary antibody. They were added in the overlying collagen gel for another experiment (see Fig. ).
    Cav3 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cav3 2/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    cav3 2 - by Bioz Stars, 2026-02
    93/100 stars
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    anti cav3 2 protein  (Alomone Labs)
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    Alomone Labs anti cav3 2 protein
    All experiments in ( a – c ) are performed at E11.5 at the ileo-cecal junction. Error bars are ±SD. a CA change relative to control before drug application for EDTA (2 mM, n = 6), GdCl3 (100 µM, n = 6), 2-APB (100 µM, n = 5), ryanodine (10 µM, n = 5). b CA change for nifedipine (Nif, 10 µM, n = 5), ruthenium red (RuR, 100 µM, n = 6), Z944 at 1 µM ( n = 7), 10 µM ( n = 7), 50 µM ( n = 9), ascorbic acid (AA, 1 mM, n = 13) and SAK3 at 1 µM ( n = 6), 10 µM ( n = 6). c CA change for NPPB (100 µM, n = 11), NFA (50 µM, n = 9) and DIDS (500 µM, n = 5). Two-tailed p -values for (a-c) are calculated from the Student t-test. Sox10 and <t>CaV3.1</t> ( d <t>),</t> <t>CaV3.2</t> ( e ) and CaV3.3 ( f ) IHCs of ENCCs migrating from an intestinal explant on a substrate. All three T-type Ca 2+ channels are expressed by ENCCs, but not by the surrounding mesenchymal cells. The small bright green dots are AF647 µ-beads fluorescing in the same range as the T-type Ca 2+ channel secondary antibody. They were added in the overlying collagen gel for another experiment (see Fig. ).
    Anti Cav3 2 Protein, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cav3 2 protein/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    anti cav3 2 protein - by Bioz Stars, 2026-02
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    rabbit  (Alomone Labs)
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    Alomone Labs rabbit
    All experiments in ( a – c ) are performed at E11.5 at the ileo-cecal junction. Error bars are ±SD. a CA change relative to control before drug application for EDTA (2 mM, n = 6), GdCl3 (100 µM, n = 6), 2-APB (100 µM, n = 5), ryanodine (10 µM, n = 5). b CA change for nifedipine (Nif, 10 µM, n = 5), ruthenium red (RuR, 100 µM, n = 6), Z944 at 1 µM ( n = 7), 10 µM ( n = 7), 50 µM ( n = 9), ascorbic acid (AA, 1 mM, n = 13) and SAK3 at 1 µM ( n = 6), 10 µM ( n = 6). c CA change for NPPB (100 µM, n = 11), NFA (50 µM, n = 9) and DIDS (500 µM, n = 5). Two-tailed p -values for (a-c) are calculated from the Student t-test. Sox10 and <t>CaV3.1</t> ( d <t>),</t> <t>CaV3.2</t> ( e ) and CaV3.3 ( f ) IHCs of ENCCs migrating from an intestinal explant on a substrate. All three T-type Ca 2+ channels are expressed by ENCCs, but not by the surrounding mesenchymal cells. The small bright green dots are AF647 µ-beads fluorescing in the same range as the T-type Ca 2+ channel secondary antibody. They were added in the overlying collagen gel for another experiment (see Fig. ).
    Rabbit, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
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    anti cav3 2  (Danaher Inc)
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    Danaher Inc anti cav3 2
    Primer sequences for quantitative polymerase chain reaction
    Anti Cav3 2, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    All experiments in ( a – c ) are performed at E11.5 at the ileo-cecal junction. Error bars are ±SD. a CA change relative to control before drug application for EDTA (2 mM, n = 6), GdCl3 (100 µM, n = 6), 2-APB (100 µM, n = 5), ryanodine (10 µM, n = 5). b CA change for nifedipine (Nif, 10 µM, n = 5), ruthenium red (RuR, 100 µM, n = 6), Z944 at 1 µM ( n = 7), 10 µM ( n = 7), 50 µM ( n = 9), ascorbic acid (AA, 1 mM, n = 13) and SAK3 at 1 µM ( n = 6), 10 µM ( n = 6). c CA change for NPPB (100 µM, n = 11), NFA (50 µM, n = 9) and DIDS (500 µM, n = 5). Two-tailed p -values for (a-c) are calculated from the Student t-test. Sox10 and CaV3.1 ( d ), CaV3.2 ( e ) and CaV3.3 ( f ) IHCs of ENCCs migrating from an intestinal explant on a substrate. All three T-type Ca 2+ channels are expressed by ENCCs, but not by the surrounding mesenchymal cells. The small bright green dots are AF647 µ-beads fluorescing in the same range as the T-type Ca 2+ channel secondary antibody. They were added in the overlying collagen gel for another experiment (see Fig. ).

    Journal: Nature Communications

    Article Title: Endothelin-3 and T-type Ca 2+ channels drive enteric neural crest cell calcium activity, contractility and migration

    doi: 10.1038/s41467-025-68121-5

    Figure Lengend Snippet: All experiments in ( a – c ) are performed at E11.5 at the ileo-cecal junction. Error bars are ±SD. a CA change relative to control before drug application for EDTA (2 mM, n = 6), GdCl3 (100 µM, n = 6), 2-APB (100 µM, n = 5), ryanodine (10 µM, n = 5). b CA change for nifedipine (Nif, 10 µM, n = 5), ruthenium red (RuR, 100 µM, n = 6), Z944 at 1 µM ( n = 7), 10 µM ( n = 7), 50 µM ( n = 9), ascorbic acid (AA, 1 mM, n = 13) and SAK3 at 1 µM ( n = 6), 10 µM ( n = 6). c CA change for NPPB (100 µM, n = 11), NFA (50 µM, n = 9) and DIDS (500 µM, n = 5). Two-tailed p -values for (a-c) are calculated from the Student t-test. Sox10 and CaV3.1 ( d ), CaV3.2 ( e ) and CaV3.3 ( f ) IHCs of ENCCs migrating from an intestinal explant on a substrate. All three T-type Ca 2+ channels are expressed by ENCCs, but not by the surrounding mesenchymal cells. The small bright green dots are AF647 µ-beads fluorescing in the same range as the T-type Ca 2+ channel secondary antibody. They were added in the overlying collagen gel for another experiment (see Fig. ).

    Article Snippet: After fixation, blocking and permeation, samples were incubated for 1 day in 1:200 mouse Anti-SOX10 and 1:100 rabbit anti CaV3.1 (Alomone Labs #ACC-021) or 1:100 rabbit anti CaV3.2 (Alomone Labs #ACC-025) or 1:100 rabbit anti CaV3.3 (Alomone Labs #ACC-009) for 24 h, washed 3 times, incubated in secondary 1:500 anti-mouse Cy3 and 1:500 anti-rabbit AF647 antibody for another day, washed and imaged.

    Techniques: Control, Two Tailed Test

    Primer sequences for quantitative polymerase chain reaction

    Journal: Neural Regeneration Research

    Article Title: Cav3.2 channel regulates cerebral ischemia/reperfusion injury: a promising target for intervention

    doi: 10.4103/1673-5374.390966

    Figure Lengend Snippet: Primer sequences for quantitative polymerase chain reaction

    Article Snippet: We incubated the membranes with primary antibodies against rabbit anti-Cav3.2 (1:500, Abcam, Cat# ab135974, RRID: AB_2892219), rabbit anti-Bcl-2 (1:2000, Abcam, Cat# ab182858, RRID: AB_2715467), rabbit anti-Bax (1:1000, Abcam, Cat# ab32503, RRID: AB_725631), rabbit anti-Lamin B (1:1000, Abcam, Cat# ab16048, RRID: AB_443298), rabbit anti-calcineurin (1:1000, Abcam, Cat# ab282104, RRID: AB_2168466), rabbit anti-nuclear factor of activated T cells 3 (NFAT3, 1:1000, Abcam, Cat# ab183117, RRID: AB_10697015) and rabbit anti-β-actin (1:1000, Abcam, Cat# ab8227, RRID: AB_2305186) overnight at 4°C and then with a goat anti-rabbit hemoglobin antibody (1:10 000, Abcam, Cat# ab205718, RRID: AB_2819160) at 37°C for 2 hours.

    Techniques: Sequencing

    Cav3.2 expression is specifically upregulated in hippocampal neurons after cerebral ischemia/reperfusion injury. (A) Cav3.2 protein and mRNA expression on the ischemic side in hippocampal tissue in WT mice ( n = 5). (B) Cav3.2 protein and mRNA expression on the ischemic side in cortical tissue in WT mice ( n = 5). (C) Cav3.2 protein and mRNA expression in primary hippocampal neurons after H/R treatment ( n = 5). Data are presented as the mean ± SD. * P < 0.05 (Student’s t -test). H/R: Hypoxia/reoxygenation; MCAO: middle cerebral artery occlusion; ns: not significant; PBS: phosphate buffered saline.

    Journal: Neural Regeneration Research

    Article Title: Cav3.2 channel regulates cerebral ischemia/reperfusion injury: a promising target for intervention

    doi: 10.4103/1673-5374.390966

    Figure Lengend Snippet: Cav3.2 expression is specifically upregulated in hippocampal neurons after cerebral ischemia/reperfusion injury. (A) Cav3.2 protein and mRNA expression on the ischemic side in hippocampal tissue in WT mice ( n = 5). (B) Cav3.2 protein and mRNA expression on the ischemic side in cortical tissue in WT mice ( n = 5). (C) Cav3.2 protein and mRNA expression in primary hippocampal neurons after H/R treatment ( n = 5). Data are presented as the mean ± SD. * P < 0.05 (Student’s t -test). H/R: Hypoxia/reoxygenation; MCAO: middle cerebral artery occlusion; ns: not significant; PBS: phosphate buffered saline.

    Article Snippet: We incubated the membranes with primary antibodies against rabbit anti-Cav3.2 (1:500, Abcam, Cat# ab135974, RRID: AB_2892219), rabbit anti-Bcl-2 (1:2000, Abcam, Cat# ab182858, RRID: AB_2715467), rabbit anti-Bax (1:1000, Abcam, Cat# ab32503, RRID: AB_725631), rabbit anti-Lamin B (1:1000, Abcam, Cat# ab16048, RRID: AB_443298), rabbit anti-calcineurin (1:1000, Abcam, Cat# ab282104, RRID: AB_2168466), rabbit anti-nuclear factor of activated T cells 3 (NFAT3, 1:1000, Abcam, Cat# ab183117, RRID: AB_10697015) and rabbit anti-β-actin (1:1000, Abcam, Cat# ab8227, RRID: AB_2305186) overnight at 4°C and then with a goat anti-rabbit hemoglobin antibody (1:10 000, Abcam, Cat# ab205718, RRID: AB_2819160) at 37°C for 2 hours.

    Techniques: Expressing, Saline

    Cav3.2 KO improves the cognitive function in cerebral ischemia/reperfusion injury mice. (A, B) The infarct volumes of brains by 2,3,5-triphenyltetrazolium chloride staining ( n = 8). Infarcted tissue appears white and normal tissue appears red. Scale bar: 2 mm. (C) The brain water content ( n = 8). (D–G) Neurological deficits were assessed with the Longa score (D), rotarod test (E), foot fault test (F), and adhesive removal test (G) ( n = 8). Data are presented as the mean ± SD. * P < 0.05 (one-way analysis of variance followed by Tukey’s multiple comparison post hoc test [B, C, E–G] or Mann-Whitney U test [D]). KO: Knockout; MCAO: middle cerebral artery occlusion; WT: wild type.

    Journal: Neural Regeneration Research

    Article Title: Cav3.2 channel regulates cerebral ischemia/reperfusion injury: a promising target for intervention

    doi: 10.4103/1673-5374.390966

    Figure Lengend Snippet: Cav3.2 KO improves the cognitive function in cerebral ischemia/reperfusion injury mice. (A, B) The infarct volumes of brains by 2,3,5-triphenyltetrazolium chloride staining ( n = 8). Infarcted tissue appears white and normal tissue appears red. Scale bar: 2 mm. (C) The brain water content ( n = 8). (D–G) Neurological deficits were assessed with the Longa score (D), rotarod test (E), foot fault test (F), and adhesive removal test (G) ( n = 8). Data are presented as the mean ± SD. * P < 0.05 (one-way analysis of variance followed by Tukey’s multiple comparison post hoc test [B, C, E–G] or Mann-Whitney U test [D]). KO: Knockout; MCAO: middle cerebral artery occlusion; WT: wild type.

    Article Snippet: We incubated the membranes with primary antibodies against rabbit anti-Cav3.2 (1:500, Abcam, Cat# ab135974, RRID: AB_2892219), rabbit anti-Bcl-2 (1:2000, Abcam, Cat# ab182858, RRID: AB_2715467), rabbit anti-Bax (1:1000, Abcam, Cat# ab32503, RRID: AB_725631), rabbit anti-Lamin B (1:1000, Abcam, Cat# ab16048, RRID: AB_443298), rabbit anti-calcineurin (1:1000, Abcam, Cat# ab282104, RRID: AB_2168466), rabbit anti-nuclear factor of activated T cells 3 (NFAT3, 1:1000, Abcam, Cat# ab183117, RRID: AB_10697015) and rabbit anti-β-actin (1:1000, Abcam, Cat# ab8227, RRID: AB_2305186) overnight at 4°C and then with a goat anti-rabbit hemoglobin antibody (1:10 000, Abcam, Cat# ab205718, RRID: AB_2819160) at 37°C for 2 hours.

    Techniques: Staining, Adhesive, Comparison, MANN-WHITNEY, Knock-Out

    Cav3.2 KO suppresses oxidative stress in the hippocampus after cerebral ischemia/reperfusion injury. (A) Quantification of 8-OHdG expression (red, stained with Alexa Fluor® 647) in the ischemic hippocampus by immunofluorescence staining ( n = 8). Double-positive 8-OHdG (red) and DAPI staining (blue) indicates the 8-OHdG-positive neurons. More positive cells were observed in the MCAO + WT group than in the sham + WT group. Fewer positive cells were observed in the MCAO + Cav3.2 KO group than in the MCAO + WT group. Scale bar: 25 μm. (B–D) Quantification of GSH-Px (B) and SOD (C) activity and MDA levels (D) in the ischemic hippocampus by biochemical anaylsis ( n = 8). All data were normalized to the mean value of the sham + WT group. Data are presented as the mean ± SD. * P < 0.05 (one-way analysis of variance followed by Tukey’s multiple comparison post hoc test). 8-OHdG: 8-Oxo-deoxyguanosine; DAPI: 4′,6-diamidino-2-phenylindole; GSH-Px: glutathione peroxidase; KO: knockout; MCAO: middle cerebral artery occlusion; MDA: malondialdehyde; SOD: superoxide dismutase; WT: wild type.

    Journal: Neural Regeneration Research

    Article Title: Cav3.2 channel regulates cerebral ischemia/reperfusion injury: a promising target for intervention

    doi: 10.4103/1673-5374.390966

    Figure Lengend Snippet: Cav3.2 KO suppresses oxidative stress in the hippocampus after cerebral ischemia/reperfusion injury. (A) Quantification of 8-OHdG expression (red, stained with Alexa Fluor® 647) in the ischemic hippocampus by immunofluorescence staining ( n = 8). Double-positive 8-OHdG (red) and DAPI staining (blue) indicates the 8-OHdG-positive neurons. More positive cells were observed in the MCAO + WT group than in the sham + WT group. Fewer positive cells were observed in the MCAO + Cav3.2 KO group than in the MCAO + WT group. Scale bar: 25 μm. (B–D) Quantification of GSH-Px (B) and SOD (C) activity and MDA levels (D) in the ischemic hippocampus by biochemical anaylsis ( n = 8). All data were normalized to the mean value of the sham + WT group. Data are presented as the mean ± SD. * P < 0.05 (one-way analysis of variance followed by Tukey’s multiple comparison post hoc test). 8-OHdG: 8-Oxo-deoxyguanosine; DAPI: 4′,6-diamidino-2-phenylindole; GSH-Px: glutathione peroxidase; KO: knockout; MCAO: middle cerebral artery occlusion; MDA: malondialdehyde; SOD: superoxide dismutase; WT: wild type.

    Article Snippet: We incubated the membranes with primary antibodies against rabbit anti-Cav3.2 (1:500, Abcam, Cat# ab135974, RRID: AB_2892219), rabbit anti-Bcl-2 (1:2000, Abcam, Cat# ab182858, RRID: AB_2715467), rabbit anti-Bax (1:1000, Abcam, Cat# ab32503, RRID: AB_725631), rabbit anti-Lamin B (1:1000, Abcam, Cat# ab16048, RRID: AB_443298), rabbit anti-calcineurin (1:1000, Abcam, Cat# ab282104, RRID: AB_2168466), rabbit anti-nuclear factor of activated T cells 3 (NFAT3, 1:1000, Abcam, Cat# ab183117, RRID: AB_10697015) and rabbit anti-β-actin (1:1000, Abcam, Cat# ab8227, RRID: AB_2305186) overnight at 4°C and then with a goat anti-rabbit hemoglobin antibody (1:10 000, Abcam, Cat# ab205718, RRID: AB_2819160) at 37°C for 2 hours.

    Techniques: Expressing, Staining, Immunofluorescence, Activity Assay, Comparison, Knock-Out

    Cav3.2 KO attenuates the inflammatory response after cerebral ischemia/reperfusion injury. (A–C) Quantification of TNF-α (A), IL-17 (B) and IL-1β (C) levels in the ischemic hippocampus by enzyme-linked immunosorbent assay ( n = 8). (D–F) Quantification of TNF-α (D), IL-17 (E) and IL-1β (F) mRNA levels in the ischemic hippocampus by quantitative polymerase chain reaction ( n = 8). TNF-α, IL-17 and IL-1β mRNA levels were normalized to the respective mean values of the sham + WT group. Data are presented as the mean ± SD. * P < 0.05 (one-way analysis of variance followed by Tukey’s multiple comparison post hoc test). IL: Interleukin; KO: knockout; MCAO: middle cerebral artery occlusion; TNF-α: tumor necrosis factor-α; WT: wild type.

    Journal: Neural Regeneration Research

    Article Title: Cav3.2 channel regulates cerebral ischemia/reperfusion injury: a promising target for intervention

    doi: 10.4103/1673-5374.390966

    Figure Lengend Snippet: Cav3.2 KO attenuates the inflammatory response after cerebral ischemia/reperfusion injury. (A–C) Quantification of TNF-α (A), IL-17 (B) and IL-1β (C) levels in the ischemic hippocampus by enzyme-linked immunosorbent assay ( n = 8). (D–F) Quantification of TNF-α (D), IL-17 (E) and IL-1β (F) mRNA levels in the ischemic hippocampus by quantitative polymerase chain reaction ( n = 8). TNF-α, IL-17 and IL-1β mRNA levels were normalized to the respective mean values of the sham + WT group. Data are presented as the mean ± SD. * P < 0.05 (one-way analysis of variance followed by Tukey’s multiple comparison post hoc test). IL: Interleukin; KO: knockout; MCAO: middle cerebral artery occlusion; TNF-α: tumor necrosis factor-α; WT: wild type.

    Article Snippet: We incubated the membranes with primary antibodies against rabbit anti-Cav3.2 (1:500, Abcam, Cat# ab135974, RRID: AB_2892219), rabbit anti-Bcl-2 (1:2000, Abcam, Cat# ab182858, RRID: AB_2715467), rabbit anti-Bax (1:1000, Abcam, Cat# ab32503, RRID: AB_725631), rabbit anti-Lamin B (1:1000, Abcam, Cat# ab16048, RRID: AB_443298), rabbit anti-calcineurin (1:1000, Abcam, Cat# ab282104, RRID: AB_2168466), rabbit anti-nuclear factor of activated T cells 3 (NFAT3, 1:1000, Abcam, Cat# ab183117, RRID: AB_10697015) and rabbit anti-β-actin (1:1000, Abcam, Cat# ab8227, RRID: AB_2305186) overnight at 4°C and then with a goat anti-rabbit hemoglobin antibody (1:10 000, Abcam, Cat# ab205718, RRID: AB_2819160) at 37°C for 2 hours.

    Techniques: Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Comparison, Knock-Out

    Cav3.2 KO attenuates cell apoptosis after cerebral ischemia/reperfusion injury. (A) Quantification of TUNEL-positive cells in the ischemic hippocampus by TUNEL staining ( n = 6). Double-positive TUNEL (red) and DAPI staining (blue) indicates the apoptotic cells. More positive cells were observed in the MCAO + WT group than in the sham + WT group. Fewer positive cells were observed in the MCAO + Cav3.2 KO group than in the MCAO + WT group. Scale bar: 50 μm. (B) Quantification of Bcl-2 and Bax protein expression ( n = 5). All data were normalized to the mean value of the sham + WT group. Data are presented as the mean ± SD. * P < 0.05 (one-way analysis of variance followed by Tukey’s multiple comparison post hoc test). Bax: Bcl2-associated X; Bcl-2: B-cell lymphoma-2; DAPI: 4′,6-diamidino-2-phenylindole; KO: knockout; MCAO: middle cerebral artery occlusion; TUNEL: TdT-mediated dUTP nick end labeling; WT: wild type.

    Journal: Neural Regeneration Research

    Article Title: Cav3.2 channel regulates cerebral ischemia/reperfusion injury: a promising target for intervention

    doi: 10.4103/1673-5374.390966

    Figure Lengend Snippet: Cav3.2 KO attenuates cell apoptosis after cerebral ischemia/reperfusion injury. (A) Quantification of TUNEL-positive cells in the ischemic hippocampus by TUNEL staining ( n = 6). Double-positive TUNEL (red) and DAPI staining (blue) indicates the apoptotic cells. More positive cells were observed in the MCAO + WT group than in the sham + WT group. Fewer positive cells were observed in the MCAO + Cav3.2 KO group than in the MCAO + WT group. Scale bar: 50 μm. (B) Quantification of Bcl-2 and Bax protein expression ( n = 5). All data were normalized to the mean value of the sham + WT group. Data are presented as the mean ± SD. * P < 0.05 (one-way analysis of variance followed by Tukey’s multiple comparison post hoc test). Bax: Bcl2-associated X; Bcl-2: B-cell lymphoma-2; DAPI: 4′,6-diamidino-2-phenylindole; KO: knockout; MCAO: middle cerebral artery occlusion; TUNEL: TdT-mediated dUTP nick end labeling; WT: wild type.

    Article Snippet: We incubated the membranes with primary antibodies against rabbit anti-Cav3.2 (1:500, Abcam, Cat# ab135974, RRID: AB_2892219), rabbit anti-Bcl-2 (1:2000, Abcam, Cat# ab182858, RRID: AB_2715467), rabbit anti-Bax (1:1000, Abcam, Cat# ab32503, RRID: AB_725631), rabbit anti-Lamin B (1:1000, Abcam, Cat# ab16048, RRID: AB_443298), rabbit anti-calcineurin (1:1000, Abcam, Cat# ab282104, RRID: AB_2168466), rabbit anti-nuclear factor of activated T cells 3 (NFAT3, 1:1000, Abcam, Cat# ab183117, RRID: AB_10697015) and rabbit anti-β-actin (1:1000, Abcam, Cat# ab8227, RRID: AB_2305186) overnight at 4°C and then with a goat anti-rabbit hemoglobin antibody (1:10 000, Abcam, Cat# ab205718, RRID: AB_2819160) at 37°C for 2 hours.

    Techniques: TUNEL Assay, Staining, Expressing, Comparison, Knock-Out, End Labeling

    Cav3.2 KO attenuates stress damage, inflammatory response and neuronal apoptosis after cerebral ischemia/reperfusion injury. (A–C) Quantification of SOD (A) and GSH-Px (B) activity and MDA levels (C) by biochemical analysis ( n = 6). (D–F) Quantification of TNF-α (D), IL-1β (E) and IL-17 (F) mRNA levels by quantitative polymerase chain reaction ( n = 6). (G) Quantification of Bcl-2 and Bax protein expression ( n = 5), which was normalized to the PBS + WT group. Data are presented as the mean ± SD. * P < 0.05 (one-way analysis of variance followed by Tukey’s multiple comparison post hoc test). ELISA: Enzyme-linked immunosorbent assay; GSH-Px: glutathione peroxidase; H/R: hypoxia/reoxygenation; IL: interleukin; KO: knockout; MDA: malondialdehyde; PBS: phosphate buffered saline; SOD: superoxide dismutase; TNF-α: tumor necrosis factor-α; WT: wild type.

    Journal: Neural Regeneration Research

    Article Title: Cav3.2 channel regulates cerebral ischemia/reperfusion injury: a promising target for intervention

    doi: 10.4103/1673-5374.390966

    Figure Lengend Snippet: Cav3.2 KO attenuates stress damage, inflammatory response and neuronal apoptosis after cerebral ischemia/reperfusion injury. (A–C) Quantification of SOD (A) and GSH-Px (B) activity and MDA levels (C) by biochemical analysis ( n = 6). (D–F) Quantification of TNF-α (D), IL-1β (E) and IL-17 (F) mRNA levels by quantitative polymerase chain reaction ( n = 6). (G) Quantification of Bcl-2 and Bax protein expression ( n = 5), which was normalized to the PBS + WT group. Data are presented as the mean ± SD. * P < 0.05 (one-way analysis of variance followed by Tukey’s multiple comparison post hoc test). ELISA: Enzyme-linked immunosorbent assay; GSH-Px: glutathione peroxidase; H/R: hypoxia/reoxygenation; IL: interleukin; KO: knockout; MDA: malondialdehyde; PBS: phosphate buffered saline; SOD: superoxide dismutase; TNF-α: tumor necrosis factor-α; WT: wild type.

    Article Snippet: We incubated the membranes with primary antibodies against rabbit anti-Cav3.2 (1:500, Abcam, Cat# ab135974, RRID: AB_2892219), rabbit anti-Bcl-2 (1:2000, Abcam, Cat# ab182858, RRID: AB_2715467), rabbit anti-Bax (1:1000, Abcam, Cat# ab32503, RRID: AB_725631), rabbit anti-Lamin B (1:1000, Abcam, Cat# ab16048, RRID: AB_443298), rabbit anti-calcineurin (1:1000, Abcam, Cat# ab282104, RRID: AB_2168466), rabbit anti-nuclear factor of activated T cells 3 (NFAT3, 1:1000, Abcam, Cat# ab183117, RRID: AB_10697015) and rabbit anti-β-actin (1:1000, Abcam, Cat# ab8227, RRID: AB_2305186) overnight at 4°C and then with a goat anti-rabbit hemoglobin antibody (1:10 000, Abcam, Cat# ab205718, RRID: AB_2819160) at 37°C for 2 hours.

    Techniques: Activity Assay, Real-time Polymerase Chain Reaction, Expressing, Comparison, Enzyme-linked Immunosorbent Assay, Knock-Out, Saline

    Cav3.2 KO reduces the activation of calcineurin/NFAT3 signaling pathway after cerebral ischemia/reperfusion injury. (A) Quantification of calcineurin, n-NFAT3 and c-NFAT3 expression in the ischemic hippocampus ( n = 4). Hippocampal calcineurin, n-NFAT3 and c-NFAT3 expression on the ischemic side were normalized to β-actin or lamin B in the sham + WT group. (B) Quantification of calcineurin, n-NFAT3 and c-NFAT3 expression in primary hippocampal neurons ( n = 4). Calcineurin, n-NFAT3 and c-NFAT3 expression in primary hippocampal neurons were normalized to β-actin or lamin B in the PBS + WT group. Data are presented as the mean ± SD. * P < 0.05 (one-way analysis of variance followed by Tukey’s multiple comparison post hoc test). c-NFAT3: Cytoplasmic nuclear factor of activated T cells; H/R: hypoxia/reoxygenation; KO: knockout; MCAO: middle cerebral artery occlusion; n-NFAT3: nuclear nuclear factor of activated T cells; PBS: phosphate buffered saline; WT: wild type.

    Journal: Neural Regeneration Research

    Article Title: Cav3.2 channel regulates cerebral ischemia/reperfusion injury: a promising target for intervention

    doi: 10.4103/1673-5374.390966

    Figure Lengend Snippet: Cav3.2 KO reduces the activation of calcineurin/NFAT3 signaling pathway after cerebral ischemia/reperfusion injury. (A) Quantification of calcineurin, n-NFAT3 and c-NFAT3 expression in the ischemic hippocampus ( n = 4). Hippocampal calcineurin, n-NFAT3 and c-NFAT3 expression on the ischemic side were normalized to β-actin or lamin B in the sham + WT group. (B) Quantification of calcineurin, n-NFAT3 and c-NFAT3 expression in primary hippocampal neurons ( n = 4). Calcineurin, n-NFAT3 and c-NFAT3 expression in primary hippocampal neurons were normalized to β-actin or lamin B in the PBS + WT group. Data are presented as the mean ± SD. * P < 0.05 (one-way analysis of variance followed by Tukey’s multiple comparison post hoc test). c-NFAT3: Cytoplasmic nuclear factor of activated T cells; H/R: hypoxia/reoxygenation; KO: knockout; MCAO: middle cerebral artery occlusion; n-NFAT3: nuclear nuclear factor of activated T cells; PBS: phosphate buffered saline; WT: wild type.

    Article Snippet: We incubated the membranes with primary antibodies against rabbit anti-Cav3.2 (1:500, Abcam, Cat# ab135974, RRID: AB_2892219), rabbit anti-Bcl-2 (1:2000, Abcam, Cat# ab182858, RRID: AB_2715467), rabbit anti-Bax (1:1000, Abcam, Cat# ab32503, RRID: AB_725631), rabbit anti-Lamin B (1:1000, Abcam, Cat# ab16048, RRID: AB_443298), rabbit anti-calcineurin (1:1000, Abcam, Cat# ab282104, RRID: AB_2168466), rabbit anti-nuclear factor of activated T cells 3 (NFAT3, 1:1000, Abcam, Cat# ab183117, RRID: AB_10697015) and rabbit anti-β-actin (1:1000, Abcam, Cat# ab8227, RRID: AB_2305186) overnight at 4°C and then with a goat anti-rabbit hemoglobin antibody (1:10 000, Abcam, Cat# ab205718, RRID: AB_2819160) at 37°C for 2 hours.

    Techniques: Activation Assay, Expressing, Comparison, Knock-Out, Saline

    Calcineurin overexpression abolishes the protective effects of Cav3.2 KO in vivo after cerebral ischemia/reperfusion injury. (A, B) The infarct volumes of the brain slices by 2,3,5-triphenyltetrazolium chloride staining ( n = 8). Infarcted tissue appears white and normal tissue appears red. Scale bar: 2 mm. (C) The brain water content ( n = 8). (D–G) Neurological deficits were assessed with the Longa score (D), rotarod test (E), foot fault test (F), and adhesion test (G) ( n = 8). Data are presented as the mean ± SD. * P < 0.05 (one-way analysis of variance followed by Tukey’s multiple comparison post hoc test [B, C, E–G] or Mann-Whitney U test [D]). KO: Knockout; LV-CaN-GFP: recombinant calcineurin overexpression lentiviral vector; LV-GFP: normal control vector; MCAO: middle cerebral artery occlusion; ns: not significant.

    Journal: Neural Regeneration Research

    Article Title: Cav3.2 channel regulates cerebral ischemia/reperfusion injury: a promising target for intervention

    doi: 10.4103/1673-5374.390966

    Figure Lengend Snippet: Calcineurin overexpression abolishes the protective effects of Cav3.2 KO in vivo after cerebral ischemia/reperfusion injury. (A, B) The infarct volumes of the brain slices by 2,3,5-triphenyltetrazolium chloride staining ( n = 8). Infarcted tissue appears white and normal tissue appears red. Scale bar: 2 mm. (C) The brain water content ( n = 8). (D–G) Neurological deficits were assessed with the Longa score (D), rotarod test (E), foot fault test (F), and adhesion test (G) ( n = 8). Data are presented as the mean ± SD. * P < 0.05 (one-way analysis of variance followed by Tukey’s multiple comparison post hoc test [B, C, E–G] or Mann-Whitney U test [D]). KO: Knockout; LV-CaN-GFP: recombinant calcineurin overexpression lentiviral vector; LV-GFP: normal control vector; MCAO: middle cerebral artery occlusion; ns: not significant.

    Article Snippet: We incubated the membranes with primary antibodies against rabbit anti-Cav3.2 (1:500, Abcam, Cat# ab135974, RRID: AB_2892219), rabbit anti-Bcl-2 (1:2000, Abcam, Cat# ab182858, RRID: AB_2715467), rabbit anti-Bax (1:1000, Abcam, Cat# ab32503, RRID: AB_725631), rabbit anti-Lamin B (1:1000, Abcam, Cat# ab16048, RRID: AB_443298), rabbit anti-calcineurin (1:1000, Abcam, Cat# ab282104, RRID: AB_2168466), rabbit anti-nuclear factor of activated T cells 3 (NFAT3, 1:1000, Abcam, Cat# ab183117, RRID: AB_10697015) and rabbit anti-β-actin (1:1000, Abcam, Cat# ab8227, RRID: AB_2305186) overnight at 4°C and then with a goat anti-rabbit hemoglobin antibody (1:10 000, Abcam, Cat# ab205718, RRID: AB_2819160) at 37°C for 2 hours.

    Techniques: Over Expression, In Vivo, Staining, Comparison, MANN-WHITNEY, Knock-Out, Recombinant, Plasmid Preparation